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Methods Used in Molecular Biology: Radioisotopes, Centrifugation, and Electrophoresis

Methods Used in Molecular Biology: Radioisotopes, Centrifugation, and Electrophoresis

Jan 5, 2019

Molecular biology is one part of reagent chemistry that uses instrumentos de laboratorio. The use of these instruments aims to find out more about the molecules in a compound. The definition of molecular biology at this time is the study of the functions and organizations of living bodies (organisms) in terms of the structure and regulation of molecular elements or constituent components. Molecular biology is a branch of biological science that examines biomolecules such as DNA, RNA, and proteins.

The development of molecular biology cannot be separated from various other disciplines, such as cell biology, genetics, biochemistry, organic chemistry, and biophysics. Basically, these sciences learn one and the same subject, that is living creatures, but with different approaches and points of view.

In studying molecular biology, in essence it will be related to the analysis of macromolecules. Analysis of these macromolecules can be done based on the reaction or by studying the physical structure. Some methods used in the study of molecular biology include the use of radioisotopes, centrifugation, and electrophoresis.

– Radioisotopes
Isotopes are chemical elements that have the same number of protons in their atomic nuclei, but the atomic mass (the number of prons and neutrons) is different. Some isotopes are labile and spontaneously decay which is sometimes followed by the spread of electromagnetic radiation. Atoms that have properties are called radioisotopes.

– Centrifugation
Centrifugation is used for cell fractionation or separation of parts of cells or organelles as well as molecular separation. The principle of centrifugation is based on the phenomenon that particles suspended in a container (tube) will settle to the bottom of the container due to the influence of gravity. The deposition rate will be accelerated by a centrifuge by rotating at high speed.

– Electrophoresis
Electrophoresis is a cellular molecular separation method based on its size by using an electric field which is applied to a medium containing samples to be separated. This technique can be used to analyze DNA, RNA, and protein